A CD3|CD28 microbead-based HIV-1 viral outgrowth

Author List
Yuri V Kuzmichev
Rebecca T Veenhuis
Christopher W Pohlmeyer
Caroline C Garliss
Victoria EK Walker-Sperling
Joel N Blankson

Abstract

Latently infected resting CD4 T cells represent a major barrier to HIV-1 eradication efforts. The standard assays used for measuring this reservoir induce activation of resting CD4 T cells with either phytohaemagglutinin (PHA) with irradiated feeder cells, or with anti-CD3 antibodies. We designed a study to compare the sensitivity of a new assay (based on the stimulation of CD4 T cells with anti-CD3 and anti-CD28 coated microbeads) with that of the traditional PHA and feeder-based viral outgrowth assay.

Article Category

HIV Treatment

Article Type

Original research

Posted Date

31-03-2017

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